MORPHOGENIC RESPONSE FROM LEAF EXPLANT OF Dendranthema grandiflora VAR MICROMARGARA

<p class="Default"><strong>Background:</strong> Chrysanthemum is one of the most popular landscape plants worldwide. Introducing desirable traits into this plant by conventional breeding has limitations due to cross-incompatibility. An approach problem introduction resistance or tolerance via genetic transformation. <em>In vitro</em> regeneration necessary for implementing transformation systems; therefore, morphogenesis required before any attempt. <strong>Objective</strong>: To induce from leaf explant <em>Dendranthema grandiflora</em> var Micromargara. <strong>Methodology</strong>: Micropropagation node cuttings was induced 3 cm in length 6 months-old that were used as explants produce seedlings source explants. Morphogenesis experiments done using segments 1 cm<sup>2</sup> 8-weeks-old <em>in vitro </em>seedlings. The transferred basal medium Murashige and Skoog at 4.4 gL<sup>-1</sup> supplemented with different growth regulators. During morphogenic process, leaves samples collected detect which process induced. <strong>Results: </strong>Node culture on 3.3 benzylaminopurine 2.2 µM yielded a vegetative increase 7 shoots, 2 12 per cutting, stem 9.5 cm. three ways morphogenesis: direct indirect roots organogenesis (rhizogenesis) and, shoot organogenesis. Direct rhizogenesis regulator-free (18 roots/explant). Indirect less efficient than rhizogenesis, get obtaining six cultured g L<sup>-1 </sup>supplemented 0.4 thidiazuron 4.5 2,4-dichlorophenoxyacetic acid, 9.05 acid. L<sup>-1</sup> 13.32 4.83 naphthalene acetic acid; 50% callus formed shoots (2 shoots/leaf explant). Through histological analysis it possible verify response obtained <strong>Implications:</strong> Regeneration var. Micromargara established herein will allow improvement variety through techniques mediated Agrobacterium other strategy incorporate silence genes interest plant. <strong>Conclusion</strong>: high capacity Micromargara, may provide an model further investigating mechanism without regulators.</p>